Sie suchten nach: Merck Millipore (Novagen)

Lieferant: Merck Millipore (Novagen)

Beschreibung: Die Perfect DNA™ Leitern enthalten verschiedene DNA-Arten in praktischen, einprägsamen Größen für die Agarose-Gelanalyse. Die Marker weisen einheitliche Bandenintensitäten auf und decken eine große Bandbreite von DNA-Größen ab.

Lieferant: Merck Millipore (Novagen)

Beschreibung: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Sicherheitsdatenblätter

Lieferant: Merck Millipore (Novagen)

Beschreibung: Benzonase® nuclease degrades all forms of DNA and RNA while having no proteolytic activity. Benzonase® is compatible with BugBuster® and PopCulture® protein extraction reagents and therefore can be added to these reagents to eliminate viscosity and remove nucleic acids from <i>E. coli </i>extracts.

Lieferant: Merck Millipore (Novagen)

Beschreibung: Die KOD HiFi DNA-Polymerase ist ein einzigartiges Proofreading-Enzym, das aus dem extrem thermophilen <i>Thermococcus kodakaraensis</i> KOD1 isoliert wird (mit höherer Aktivität und Genauigkeit) und somit eine schnellere und genauere PCR-Amplifizierung ermöglicht, als sie mit herkömmlichen Enzymen erreicht werden kann.

Lieferant: Merck Millipore (Novagen)

Beschreibung: GST Bind™ Resin utilizes an 14-atom spacer arm to covalently attach reduced glutathione via a sulfide linkage. The high degree of substitution of glutathione ensures a high binding capacity with yields of glutathione S-transferase (GST) fusion proteins of 5 to 8 mg/ml settled resin. The resin can be re-used up to 6 times without loss of capacity.

Lieferant: Merck Millipore (Novagen)

Beschreibung: Pellet Paint® NF Co-Precipitant ist ein nicht fluoreszierender, mit Farbstoff markierter Träger, der sich für die fluoreszierende Sequenzierung eignet. Er ermöglicht während der Alkoholfällung der Zyklussequenzierung von Reaktionsprodukten die rasche Entfernung von BigDye® Terminatoren. Zyklussequenzierungsreaktionen können mit Zentrifugationszeiten von 10 Minuten schnell gefällt werden. Der gut sichtbare Träger liefert eine einfache Bestätigung, dass die Fällung stattgefunden hat. Die Produkte der Sequenzierungsreaktion werden effizient pelletiert, und die mit Farbstoff markierten Terminatoren verbleiben bei Verwendung der Standard-Fällungsprotokolle von Applied Biosystems während der Alkoholfällung im Überstandmedium. Die Resuspension von pelletierten Sequenzierungsreaktionsprodukten in deionisiertem Formamid kann durch die Überprüfung der Auflösung der Trägerpellets bestätigt werden.

Artikel-Nr: (71840-3)

Lieferant: Merck Millipore (Novagen)

Beschreibung: The HisTag® Antibody HRP Conjugate kit contains a horseradish peroxidase-conjugated HisTag® Monoclonal Antibody that is useful as a single detection reagent, thus eliminating cross-reactivity associated with secondary antibody reagents. It recognises five consecutive histidine residues regardless of the surrounding amino acid context. The purified antibody conjugate produces a strong signal at a 1:1000 to 1:2000 dilution on Western blots and dot blots. The HisTag® Antibody HRP Conjugate also detects the recombinant marker bands in the Novagen Perfect Protein™ Western Markers for convenient visualisation of accurate internal standards on Western blots.

UOM: 1 * 125 µl

Aktion

Artikel-Nr: (71978-3)

Lieferant: Merck Millipore (Novagen)

Beschreibung: The One Step RT-PCR Master Mix Kit allows rapid, sensitive analysis of gene expression from tissues and cells. It can replace methods for detecting and quantifying gene expression such as Northern blots,<i> in situ</i> hybridisation, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilises recombinant <i>Thermus thermophilus</i> (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimised buffer, and ultrapure deoxynucleotides. Antibody-mediated Hot Start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.

UOM: 1 * 1 KIT

Aktion

Artikel-Nr: (69831-3)

Lieferant: Merck Millipore (Novagen)

Beschreibung: The Mouse Ig-Primer Set is designed for amplification of immunoglobulin (Ig) variable region cDNAs. Mammalian light- and heavy-chain Igs contain conserved regions adjacent to the hypervariable complementarity defining regions (CDRs). Appropriately designed oligonucleotide primer sets enable these regions to be specifically amplified using PCR, after which the products may be sequenced directly or cloned.

UOM: 1 * 1 SET

Aktion

Artikel-Nr: (72158-3)

Lieferant: Merck Millipore (Novagen)

Beschreibung: The Novagen® BacMagic™-2 transfection kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

UOM: 1 * 1 KIT

Aktion

Lieferant: Merck Millipore (Novagen)

Beschreibung: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.

Lieferant: Merck Millipore (Novagen)

Beschreibung: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.

Lieferant: Merck Millipore (Novagen)

Beschreibung: rLysozyme™ solution contains a highly purified and stabilised recombinant lysozyme that can be used for lysis of gram negative bacteria, such as <i>E. coli</i>.

Lieferant: Merck Millipore (Novagen)

Beschreibung: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Lieferant: Merck Millipore (Novagen)

Beschreibung: Premixed 2X KOD Hot Start PCR components for convenience and reproducibility.

Artikel-Nr: (71975-3)

Lieferant: Merck Millipore (Novagen)

Beschreibung: KOD Xtreme™ Hot Start DNA Polymerase is an ultra high fidelity DNA polymerase designed for the most challenging PCR applications, including crude sample PCR, amplification of long strand and highly GC-rich DNA templates.

UOM: 1 * 200 LE

Aktion