Sie suchten nach: Merck Millipore (Novagen)

Lieferant: Merck Millipore (Novagen)

Beschreibung: GST Bind™ Resin utilizes an 14-atom spacer arm to covalently attach reduced glutathione via a sulfide linkage. The high degree of substitution of glutathione ensures a high binding capacity with yields of glutathione S-transferase (GST) fusion proteins of 5 to 8 mg/ml settled resin. The resin can be re-used up to 6 times without loss of capacity.

Lieferant: Merck Millipore (Novagen)

Beschreibung: Pellet Paint® NF Co-Precipitant ist ein nicht fluoreszierender, mit Farbstoff markierter Träger, der sich für die fluoreszierende Sequenzierung eignet. Er ermöglicht während der Alkoholfällung der Zyklussequenzierung von Reaktionsprodukten die rasche Entfernung von BigDye® Terminatoren. Zyklussequenzierungsreaktionen können mit Zentrifugationszeiten von 10 Minuten schnell gefällt werden. Der gut sichtbare Träger liefert eine einfache Bestätigung, dass die Fällung stattgefunden hat. Die Produkte der Sequenzierungsreaktion werden effizient pelletiert, und die mit Farbstoff markierten Terminatoren verbleiben bei Verwendung der Standard-Fällungsprotokolle von Applied Biosystems während der Alkoholfällung im Überstandmedium. Die Resuspension von pelletierten Sequenzierungsreaktionsprodukten in deionisiertem Formamid kann durch die Überprüfung der Auflösung der Trägerpellets bestätigt werden.

Artikel-Nr: (71840-3)

Lieferant: Merck Millipore (Novagen)

Beschreibung: The HisTag® Antibody HRP Conjugate kit contains a horseradish peroxidase-conjugated HisTag® Monoclonal Antibody that is useful as a single detection reagent, thus eliminating cross-reactivity associated with secondary antibody reagents. It recognises five consecutive histidine residues regardless of the surrounding amino acid context. The purified antibody conjugate produces a strong signal at a 1:1000 to 1:2000 dilution on Western blots and dot blots. The HisTag® Antibody HRP Conjugate also detects the recombinant marker bands in the Novagen Perfect Protein™ Western Markers for convenient visualisation of accurate internal standards on Western blots.

UOM: 1 * 125 µl

Aktion

Artikel-Nr: (71978-3)

Lieferant: Merck Millipore (Novagen)

Beschreibung: The One Step RT-PCR Master Mix Kit allows rapid, sensitive analysis of gene expression from tissues and cells. It can replace methods for detecting and quantifying gene expression such as Northern blots,<i> in situ</i> hybridisation, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilises recombinant <i>Thermus thermophilus</i> (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimised buffer, and ultrapure deoxynucleotides. Antibody-mediated Hot Start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.

UOM: 1 * 1 KIT

Aktion

Artikel-Nr: (69831-3)

Lieferant: Merck Millipore (Novagen)

Beschreibung: The Mouse Ig-Primer Set is designed for amplification of immunoglobulin (Ig) variable region cDNAs. Mammalian light- and heavy-chain Igs contain conserved regions adjacent to the hypervariable complementarity defining regions (CDRs). Appropriately designed oligonucleotide primer sets enable these regions to be specifically amplified using PCR, after which the products may be sequenced directly or cloned.

UOM: 1 * 1 SET

Aktion

Lieferant: Merck Millipore (Novagen)

Beschreibung: This His Tag® monoclonal antibody is a mouse monoclonal antibody (IgG1) directed against the His tag sequence encoded by many of the expression vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context.

Artikel-Nr: (72157-3)

Lieferant: Merck Millipore (Novagen)

Beschreibung: The BacMagic™-2 DNA Kit provides a positive selection for creating baculoviruses recombinants in preparation for generating recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

UOM: 1 * 1 KIT

Aktion

Artikel-Nr: (71759-4)

Lieferant: Merck Millipore (Novagen)

Beschreibung: Overnight Express™ Autoinduction NMR Medium, 5 L kit

UOM: 1 * 4 SET

Aktion

Artikel-Nr: (69671-3)

Lieferant: Merck Millipore (Novagen)

Beschreibung: Restriction grade thrombin is a highly purified preparation qualified to specifically cleave target proteins produced with appropriate vectors. This preparation is functionally tested for activity with fusion proteins and is free of detectable contaminating proteases.

UOM: 1 * 50 EU

Aktion

Lieferant: Merck Millipore (Novagen)

Beschreibung: ColiRollers™ plating beads are a specially treated glass bead which offers a better way to perform plating bacteria for transformation and molecular cloning applications. ColiRollers™ beads eliminate the inconsistencies of conventional 'hockey stick' methods.

Sicherheitsdatenblätter

Artikel-Nr: (69182-3)

Lieferant: Merck Millipore (Novagen)

Beschreibung: Desoxyribonuclease I

UOM: 1 * 1.000 EU

Aktion

Lieferant: Merck Millipore (Novagen)

Beschreibung: BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.

Artikel-Nr: (69067-3)

Lieferant: Merck Millipore (Novagen)

Beschreibung: The Enterokinase Cleavage Capture Kit is designed for highly specific cleavage of fusion proteins followed by rapid, affinity-based capture and removal of enterokinase. Following cleavage of the target protein, rEK is removed with >99% efficiency from the reaction by affinity capture on EKapture™ Agarose. Following capture of rEK, the EKapture Agarose is removed by spin filtration, because the same buffer conditions are used for both cleavage and capture no buffer changes are necessary.

UOM: 1 * 1 KIT

Aktion

Artikel-Nr: (69278-3)

Lieferant: Merck Millipore (Novagen)

Beschreibung: Die PCR-Marker enthalten eine Mischung aus acht definierten DNA-Fragmenten in praktischen Größenstufen von 50 bis 2000 bp zur Charakterisierung kleiner DNA-Produkte. Die Marker werden in einem Gelladepuffer mit zwei Farben zur Verfolgung geliefert, welche die Fluoreszenz der Ethidiumbromid-gefärbten Banden nicht beeinträchtigen. Ein separates Röhrchen mit 6X Ladepuffer ist ebenfalls im Lieferumfang enthalten. Jedes Markerröhrchen reicht für 50 Bahnen auf Ethidiumbromid-gefärbter TBE- oder TAE-Agarose. Die empfohlene Lademenge (5 µl) ergibt Banden von gleichmäßiger Intensität, die hell, scharf und einfach zu fotografieren sind.

UOM: 1 * 1 ST

Aktion

Lieferant: Merck Millipore (Novagen)

Beschreibung: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Artikel-Nr: (69909-3)

Lieferant: Merck Millipore (Novagen)

Beschreibung: The pET-30a-c(+) vectors carry an N-terminal His Tag®/thrombin/S Tag™/enterokinase configuration plus an optional C-terminal His tag sequence.

UOM: 1 * 10 µG

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