Sie suchten nach: Merck Millipore (Novagen)

Lieferant: Merck Millipore (Novagen)

Beschreibung: BugBuster® master mix combines BugBuster® protein extraction reagent with Benzonase® Nuclease and rLysozyme™ solution in one convenient reagent. It allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The master mix provides sufficient reagents for protein extraction from 20 g and 100 g cell paste.

Artikel-Nr: (71366-4)

Lieferant: Merck Millipore (Novagen)

Beschreibung: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.

UOM: 1 * 1 ST

Aktion

Lieferant: Merck Millipore (Novagen)

Beschreibung: BugBuster® 10X is a concentrated formulation of the proprietary detergents employed in BugBuster® without the addition of salts or buffer components. Concentrated BugBuster® provides a flexible alternative to the ready-to-use standard 1X BugBuster®, allowing user-defined dilution and addition of buffer components. BugBuster® 10X has all of the bioprocessing benefits of standard BugBuster® plus the freedom to control pH, reagent concentration, and buffer additives necessary for maximum extraction and activity of your target protein.

Lieferant: Merck Millipore (Novagen)

Beschreibung: The CytoBuster™ protein extraction reagent is a proprietary formulation of detergents optimised for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of the reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw.

Lieferant: Merck Millipore (Novagen)

Beschreibung: Novagen® T7Select® Phage Display System takes advantage of the properties of bacteriophage T7. This system is easy to use and has the capacity to display peptides up to about 50 amino acids in size in high copy number (415 per phage), and peptides or proteins up to about 1200 amino acids in low copy number (0,1 to 1 per phage) or mid-copy number (5 to 15 per phage).

Lieferant: Merck Millipore (Novagen)

Beschreibung: Das Transfektionsreagenz Insect GeneJuice® ist für höchste Transfektionseffizienz bei Sf9-Insektenzellen formuliert. Das Reagenz zeichnet sich durch seine äußerst niedrige Zelltoxizität aus und kann sowohl bei transienten als auch stabilen Transfektionen in serumhaltigen oder serumfreien Medien verwendet werden. Ferner eignet es sich zur Kotransfektion von Transferplasmiden mit linearisierter Virus-DNA für die Herstellung von rekombinanten Baculoviren.

Lieferant: Merck Millipore (Novagen)

Beschreibung: THP [Tris(hydroxypropyl)phosphine; C9H21O3P] is a water-soluble, odorless, sulfhydryl reducing agent that is more stable and effective than dithiothreitol (DTT).

Lieferant: Merck Millipore (Novagen)

Beschreibung: Lysonase™ bioprocessing reagent is an optimised, ready-to-use convenient blend of rLysozyme™ solution and benzonase nuclease which is ideal for protein extraction.

Sicherheitsdatenblätter

Lieferant: Merck Millipore (Novagen)

Beschreibung: A common method for transformation of plasmids into <i>E.coli</i> is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Artikel-Nr: (69959-3)

Lieferant: Merck Millipore (Novagen)

Beschreibung: The Perfect Protein™ Western Markers consist of seven recombinant S•Tag™/His•Tag® fusion proteins that can be detected on any Western blot using an S-protein AP or HRP conjugate. As an additional option, the markers can also be detected with an His•Tag Monoclonal Antibody and an AP or HRP conjugated secondary antibody. The Perfect Protein Western Markers serve as precise size standards that appear simultaneously on the blot with target proteins, eliminating the uncertainty and imprecision associated with other methods.

UOM: 1 * 1 ST

Aktion

Lieferant: Merck Millipore (Novagen)

Beschreibung: Biotinylated Thrombin has covalently attached biotin for easy removal of the enzyme from cleavage reactions using immobilised streptavidin. This preparation is tested for activity using the same assay as for unmodified thrombin, and for >99% binding to streptavidin agarose.

Lieferant: Merck Millipore (Novagen)

Beschreibung: Die Perfect DNA™ Leitern enthalten verschiedene DNA-Arten in praktischen, einprägsamen Größen für die Agarose-Gelanalyse. Die Marker weisen einheitliche Bandenintensitäten auf und decken eine große Bandbreite von DNA-Größen ab.

Lieferant: Merck Millipore (Novagen)

Beschreibung: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Sicherheitsdatenblätter

Lieferant: Merck Millipore (Novagen)

Beschreibung: Benzonase® nuclease degrades all forms of DNA and RNA while having no proteolytic activity. Benzonase® is compatible with BugBuster® and PopCulture® protein extraction reagents and therefore can be added to these reagents to eliminate viscosity and remove nucleic acids from <i>E. coli </i>extracts.

Lieferant: Merck Millipore (Novagen)

Beschreibung: Die KOD HiFi DNA-Polymerase ist ein einzigartiges Proofreading-Enzym, das aus dem extrem thermophilen <i>Thermococcus kodakaraensis</i> KOD1 isoliert wird (mit höherer Aktivität und Genauigkeit) und somit eine schnellere und genauere PCR-Amplifizierung ermöglicht, als sie mit herkömmlichen Enzymen erreicht werden kann.

Lieferant: Merck Millipore (Novagen)

Beschreibung: GST Bind™ Resin utilizes an 14-atom spacer arm to covalently attach reduced glutathione via a sulfide linkage. The high degree of substitution of glutathione ensures a high binding capacity with yields of glutathione S-transferase (GST) fusion proteins of 5 to 8 mg/ml settled resin. The resin can be re-used up to 6 times without loss of capacity.