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Sie suchten nach: Reagenzien für Sequentierung

Sequencing reagents facilitate the processes when determining the precise order of nucleotides within DNA strands through sorting the fragments based on size. Enzymes are optimized to work together for high-efficiency removal of unincorporated primers and nucleotides. These reagents produce exceptional sequencing results from difficult templates, such as those with high GC content and secondary structures. Essential for basic and applied research procedures, sequencing reagents allow researchers to discover genotypes and phenotypes of specimen.

Sequencing reagents facilitate the processes when determining the precise order of nucleotides within DNA strands through sorting the fragments based on size. Enzymes are optimized to work together for high-efficiency removal of unincorporated primers and nucleotides. These reagents produce exceptional sequencing results from difficult templates, such as those with high GC content and secondary structures. Essential for basic and applied research procedures, sequencing reagents allow researchers to discover genotypes and phenotypes of specimen.


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Product Image-MAG-FRAG-I-5

Fragment selection kits, Axygen® AxyPrep™ FragmentSelect-I Kit

Artikel-Nr: (MAG-FRAG-I-5)
Lieferant: Corning
Beschreibung: The AxyPrep™ FragmentSelect Kits utilise a paramagnetic bead technology for quick high throughput optimised DNA size selection suitable for various next generation sequencing platforms. The AxyPrep™ FragmentSelect-I kit is optimised for Illumina next generation sequencing platforms and Life Technologies (SOLiD™) fragment size selection needs simplifying the library construction workflow.
UOM: 1 * 1 KIT
Product Image-AATB17066

MagaDye™ 561 ddATP

Lieferant: AAT BIOQUEST
Beschreibung: Die Sanger-Sequenzierung, auch als Kettenabbruch-Synthese bekannt, ist eine Technik zur DNA-Sequenzierung, die auf dem selektiven Einbau kettenterminierender Didesoxynukleotide (ddNTPs) durch DNA-Polymerase basiert.

Product Image-AATB17208

ddTTP [2',3'-Dideoxythymidine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)

Artikel-Nr: (AATB17208)
Lieferant: AAT BIOQUEST
Beschreibung: Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
UOM: 1 * 1 µmol
Product Image-AATB17067

MagaDye™ 535 ddGTP

Lieferant: AAT BIOQUEST
Beschreibung: Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

Product Image-AATB17207

ddCTP [2',3'-Dideoxycytidine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)

Artikel-Nr: (AATB17207)
Lieferant: AAT BIOQUEST
Beschreibung: Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
UOM: 1 * 1 µmol
Product Image-AATB17210

ddGTP [2',3'-Dideoxyguanosine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)

Artikel-Nr: (AATB17210)
Lieferant: AAT BIOQUEST
Beschreibung: Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
UOM: 1 * 1 µmol
Product Image-AATB17080

2-Aminoethoxypropargyl ddCTP (1 µmole)

Artikel-Nr: (AATB17080)
Lieferant: AAT BIOQUEST
Beschreibung: Sanger method is one of the most reliable and earliest DNA sequencing methods.
UOM: 1 * 1 µmol
Product Image-AATB17064

MagaDye™ 613 ddCTP

Lieferant: AAT BIOQUEST
Beschreibung: Die Sanger-Sequenzierung, auch als Kettenabbruch-Synthese bekannt, ist eine Technik zur DNA-Sequenzierung, die auf dem selektiven Einbau kettenterminierender Didesoxynukleotide (ddNTPs) durch DNA-Polymerase basiert.

Product Image-AATB17086

2-Aminoethoxypropargyl ddGTP (1 µmole)

Artikel-Nr: (AATB17086)
Lieferant: AAT BIOQUEST
Beschreibung: Sanger method is one of the most reliable and earliest DNA sequencing methods.
UOM: 1 * 1 µmol
Product Image-AATB17065

MagaDye™ 588 ddTTP

Lieferant: AAT BIOQUEST
Beschreibung: Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

Product Image-AATB17082

2-Aminoethoxypropargyl ddTTP (1 µmole)

Artikel-Nr: (AATB17082)
Lieferant: AAT BIOQUEST
Beschreibung: Sanger method is one of the most reliable and earliest DNA sequencing methods.
UOM: 1 * 1 µmol
Product Image-AATB17084

2-Aminoethoxypropargyl ddATP (1 µmole)

Artikel-Nr: (AATB17084)
Lieferant: AAT BIOQUEST
Beschreibung: Sanger method is one of the most reliable and earliest DNA sequencing methods.
UOM: 1 * 1 µmol
Product Image-AATB17209

ddATP [2',3'-Dideoxyadenosine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)

Artikel-Nr: (AATB17209)
Lieferant: AAT BIOQUEST
Beschreibung: Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
UOM: 1 * 1 µmol
Image Unavailable-786-15

Glutamic-C™, sequencing grade

Artikel-Nr: (786-15)
Lieferant: G-Biosciences
Beschreibung: SG-Glutamic-C™ is a serine endopeptidase, from<i> S. aureus V8</i>, that is highly specific for the cleavage of peptide bonds at the carboxy side of either aspartic or glutamic acid, depending on the buffer used. In Tris-HCl buffer, in particular in the absence of phosphate ions, the enzyme is specific for the glutamyl site. Recommended buffers for fragmentation of proteins using this enzyme are 50 mM Tris-HCI, pH 8,0 or bicarbonate buffer. Highly purified preparations of SG-Glutamic-C™ are chemically modified making the enzyme both resistant to autolysis and stabilises its enzymatic activity.
UOM: 1 * 2 ST
Image Unavailable-786-11

Arginine-C™, sequencing grade

Artikel-Nr: (786-11)
Lieferant: G-Biosciences
Beschreibung: SG-Arginine-C™ endopeptidase (Clostripain, from <i>C. histolyticum</i>) specifically hydrolyses the carboxy peptide bond of arginine. SG-Arginine-C™ has been modified chemically by a propriety process to render the enzyme resistant to autolysis and stabilise enzymatic activity. In addition, as a sulfhydryl enzyme, SG-Arginine-C™ is susceptible to inactivation by oxidation and as a result requires reducing agents for protection. The enzyme also requires calcium ion for maximal activity. A special reconstitution buffer is supplied, which contains reducing agents and activators to maintain enzyme activity.
UOM: 1 * 2 ST
Image Unavailable-786-13

Chymotrypsin™, sequencing grade

Artikel-Nr: (786-13)
Lieferant: G-Biosciences
Beschreibung: SG-Chymotrypsin™ is a serine endopeptidase, which predominantly cleaves peptide bonds on the carboxy side of tyrosine, phenylalanine and tryptophan. In addition, chymotrypsin has a low catalytic activity against the carboxy side of leucine, methionine, alanine, aspartic and glutamic acids. It is therefore recommended to always use the shortest digestion time possible.
UOM: 1 * 2 ST
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