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Sequencing reagents facilitate the processes when determining the precise order of nucleotides within DNA strands through sorting the fragments based on size. Enzymes are optimized to work together for high-efficiency removal of unincorporated primers and nucleotides. These reagents produce exceptional sequencing results from difficult templates, such as those with high GC content and secondary structures. Essential for basic and applied research procedures, sequencing reagents allow researchers to discover genotypes and phenotypes of specimen.