Sie suchten nach: Biowest

Lieferant: Biowest

Beschreibung: Minimum Essential Medium (MEM) with Earle's Balanced Salts is a modification of Eagle's earlier medium, Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. These media have demonstrated the ability to support a variety of normal and transformed cells in culture and contain Earle's Balanced Salts, which make them suitable for use in atmospheres charged with CO₂ gas.

Sicherheitsdatenblätter Zertifikate

Lieferant: Biowest

Beschreibung: Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge the cells from their substrate depends on the sensitivity of the cells.

Sicherheitsdatenblätter Zertifikate

Artikel-Nr: (L0014-100)

Lieferant: Biowest

Beschreibung: Glutamine penicillin streptamycin acts against gram-negative and gram-positive bacteria.

UOM: 1 * 100 mL

Zertifikate Aktion

Lieferant: Biowest

Beschreibung: Ham's F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. It is frequently used with dialysed serum, hormones, selenium and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat and has proved to be an excellent cloning medium for the culture of myeloma and hybrid cells.

Sicherheitsdatenblätter Zertifikate

Lieferant: Biowest

Beschreibung: Studies to determine the nutritional requirements of many cells have been in progress since Eagle's first reports. The major essential nutrients were identified, and work became focussed on the media requirements of individual cell types. Many media designed for these purposes are now available. Among the first of these media, developed initially to study hormonal requirements of cells in culture, was a mixture of DMEM and Ham's F12 medium, known as DMEM-Ham's F12.

Sicherheitsdatenblätter Zertifikate

Lieferant: Biowest

Beschreibung: RPMI are general purpose enriched media with extensive applications for a broad spectrum of mammalian and hybridoma cells, including human myeloma, mouse hybridoma, human leukocytes, and B and T lymphocytes. It was originally formulated for suspension cultures and monolayer culture of human leukaemia cells.

Sicherheitsdatenblätter Zertifikate

Lieferant: Biowest

Beschreibung: Antibiotic G418 is an aminoglycoside antibiotic similar in structure to gentamycin. It exhibits toxicity towards both eukaryotic and prokaryotic cells. The optimal concentration for selection and maintenance must be determined for each cell line.

Sicherheitsdatenblätter Zertifikate

Artikel-Nr: (L0207-500)

Lieferant: Biowest

Beschreibung: Schneider’s Insect Medium was developed to support the growth of excised imaginal discs from the fruit fly, <i>Drosophila melanogaster</i>. When supplemented with 5 to 20% heat-inactivated foetal bovine serum Schneider's mediuam has been found to support the rapid growth of both primary and established cultures of cells derived from Drosophila spp. and several other dipterans.

UOM: 1 * 500 mL

Sicherheitsdatenblätter Zertifikate Aktion

Artikel-Nr: (L0950-100)

Lieferant: Biowest

Beschreibung: Accustase is a ready to use cell detachment solution of proteolytic and collagenolytic enzymes. Accutase is a direct gentle replacement for trypsin solution. It can also be used on suspension cells to reduce their agglutination in order to ease counting.

UOM: 1 * 100 mL

Sicherheitsdatenblätter Zertifikate Aktion

Lieferant: Biowest

Beschreibung: Lymphosep is a standardised, high quality medium designed for simple and rapid isolation of PBMCs from treated whole blood. Lymphosep, like Ficoll™, has the optimised density 1,077 g/ml for lymphocyte separation to guarantee best and reliable results for your preparations.

Zertifikate

Lieferant: Biowest

Beschreibung: Gentamicin sulfate acts against gram-negative, gram-positive bacteria and mycoplasma.

Sicherheitsdatenblätter Zertifikate

Lieferant: Biowest

Beschreibung: Dulbecco's Modified Eagle Medium (DMEM) is a modification of Basal Medium Eagle (BME) which contains approximately four times as much of the vitamins and amino acids, and two to four times as much glucose (depending on the version) as the original formulation.

Sicherheitsdatenblätter Zertifikate

Lieferant: Biowest

Beschreibung: L-Glutamin ist eine essentielle Aminosäure, die von praktisch allen in Kultur gezüchteten Säugetier- und Insektenzellen benötigt wird. Es ist ein entscheidender Bestandteil vieler Zellkulturmedien und dient als wichtige Energiequelle für Zellen in Kultur. L-Glutamin ist als Trockenpulver und als gefrorene Lösung sehr stabil. In flüssigen Medien oder Stammlösungen kann L-Glutamin jedoch relativ schnell abgebaut werden. L-Glutamin ist labiler in Zellkulturlösungen als andere Aminosäuren.

Sicherheitsdatenblätter Zertifikate

Lieferant: Biowest

Beschreibung: Penicillin-Streptomycin acts against Gram-negative and Gram-positive bacteria.

Sicherheitsdatenblätter Zertifikate

Artikel-Nr: (L0630-100)

Lieferant: Biowest

Beschreibung: Versene can be used instead of trypsin. It is a chelating agent that disperses the cells by cutting the cytoplasmic bridges between them. Versene has the advantage of being able to withstand autoclave temperature, which is a guarantee of sterility. In addition, it is less aggressive cells than trypsin, which is useful for studies on cell growth.

UOM: 1 * 100 mL

Sicherheitsdatenblätter Zertifikate Aktion

Lieferant: Biowest

Beschreibung: These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells <i>in vitro</i>. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.

Zertifikate