Sie suchten nach: Western Blotting Reagents

Lieferant: Thermo Fisher Scientific

Beschreibung: The Pierce™ Chemiluminescent nucleic acid detection module is a complete system for the detection of biotin-labeled nucleic acids for various blotting applications, including Northern/Southern blots, ribonuclease protection assays (RPAs), and electrophoretic mobility shift assays (EMSAs) or gel-shift assays.

Artikel-Nr: (PIER15168)

Lieferant: Thermo Fisher Scientific

Beschreibung: SuperSignal™ West HisProbe Kit contains HisProbe-HRP conjugate and reagents required for detection of polyhistidine-tagged protein by Western blotting. HisProbe-HRP Conjugate is a nickel (Ni²⁺)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins.

UOM: 1 * 1 KIT

Aktion

Artikel-Nr: (PIER34065)

Lieferant: Thermo Fisher Scientific

Beschreibung: The Pierce™ Metal enhanced DAB substrate kit uses cobalt and nickel chloride in a special formulation of diaminobenzidine peroxidase substrate that yields intense colour for immunohistochemical staining.

UOM: 1 * 250 mL

Aktion

Lieferant: G-Biosciences

Beschreibung: Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis. The presence of contaminants, or interfering agents, such as salts, acids, bases and detergents, result in band distortion and poor protein resolution. As a result, SDS-PAGE gels are hard to analyse and lack reproducibility. PAGE-Perfect™ is a simple, two-step method for concentrating, cleaning and preparing protein solutions for running publication quality gels.

Artikel-Nr: (69078-3)

Lieferant: Merck Millipore (Novagen)

Beschreibung: The Perfect Protein™ HRP Western Blot Kit includes an HRP or AP conjugate in addition to Perfect Protein™ Western markers.

UOM: 1 * 1 ST

Aktion

Lieferant: Thermo Fisher Scientific

Beschreibung: SSC solution is commonly used in nucleic acid hybridization techniques at concentrations ranging from 0,1X to 20X depending on the application. It is used to facilitate transfer of nucleic acids to membranes in Southern and Northern blotting, dot blotting, and colony and plaque lifts.

Sicherheitsdatenblätter Zertifikate

Lieferant: Rockland Immunochemicals

Beschreibung: FemtoMax™ is an extremely sensitive, non radioactive, and enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP). It allows for the detection of femtogram (10 to 15) amounts of antigen using photographic film or other imaging methods including highly sensitive CCD cameras.

Lieferant: Thermo Fisher Scientific

Beschreibung: The Fast Western Blot Kit, SuperSignal™ West Pico Substrate contains optimised reagents and protocol for picogram-level detection and and Western blot development less than 1 hour after gel transfer.

Lieferant: Rockland Immunochemicals

Beschreibung: TrueBlot® Western blot kits contain the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples using TrueBlot® second step immunoblotting reagents in conjunction with the user's own primary IP antibody and primary (mouse, goat or rabbit IgG) Western blotting antibody.

Sicherheitsdatenblätter

Artikel-Nr: (WB59-100)

Lieferant: Merck Millipore (Oncogene)

Beschreibung: This Western-Re-Probe™ reagent is validated for use in immunoblotting.

UOM: 1 * 100 mL

Aktion

Lieferant: Thermo Fisher Scientific

Beschreibung: The Pierce™ Fast Western Blot Kit, SuperSignal™ West Femto Substrate contains optimised reagents that shorten the time to perform a typical Western blot from 4 hours to ~60 minutes.

Artikel-Nr: (J63209.AK)

Lieferant: Thermo Fisher Scientific

Beschreibung: Saponin permeating solution, is used as a cell permeabilising agent.

UOM: 1 * 250 mL

Sicherheitsdatenblätter Zertifikate Aktion

Lieferant: G-Biosciences

Beschreibung: Perfect-FOCUS™ is based on quantitative precipitation and concentration of protein solutions using UPPA™ (Universal Protein Precipitation Agent) and has been specifically developed for preparing lower conductivity protein samples for iso-electric focusing gels. Perfect-FOCUS™ is designed to clean and remove interfering agents from protein samples and concentrate protein solutions that give poor protein spot resolution during 2D electrophoresis.

Lieferant: Thermo Fisher Scientific

Beschreibung: For chemiluminecent HRP-based detection of western blotting systems. It is soluble in most polar organic solvents but is insoluble in water.

Sicherheitsdatenblätter Zertifikate

Artikel-Nr: (A3417.5000)

Lieferant: PanReac AppliChem

Beschreibung: Das CheLuminate-HRP PicoDetect ist ein komplettes Kit mit gebrauchsfertigen Reagenzien für die Chemilumineszenz-Nachweis von immobilisierten Proteinen (Western Blot) oder immobilisierten Nukleinsäuren (Southern oder Northern Blot), die direkt oder indirekt mit Meerrettichperoxidase (HRP) konjugiert sind.

UOM: 1 * 1 KIT

Aktion

Lieferant: Rockland Immunochemicals

Beschreibung: These Chemiluminescent Western Blot Kits allow for the detection of primary polyclonal or monoclonal antibodies provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. Detection of the membrane-bound primary antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialised formulation of luminol, an extremely sensitive, non radioactive substrate that emits light and allows visualisation using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. Because of the extremely high sensitivity of the kit, primary and secondary antibodies can be used at greater dilutions. Primary antibodies currently diluted 1:1000 using an ECL™ substrate is the equivalent of a 1:5000 dilution using the FemtoMax™ substrate.