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Artikel-Nr: (69772-3)

Lieferant: Merck Millipore (Novagen)

Beschreibung: pET-24(+) is a transcription vector designed for expression from bacterial translation signals carried within a cloned insert. It lacks the ribosome binding site and ATG start codon present on the pET translation vectors. A C-terminal His·Tag® sequence is available

UOM: 1 * 10 µG

Aktion

Lieferant: Merck Millipore (Novagen)

Beschreibung: The pSTBlue-1 AccepTor™ vector kits are designed to simplify the PCR cloning process by using non-proofreading thermostable DNA polymerases (eg. KOD XL polymerase and native and recombinant <i>Taq</i> polymerases), which leave single 3'-dA overhangs on the reaction products. The AccepTor™ vectors enable direct ligation by virtue of single 3'-dU overhangs that anneal efficiently with 3'-dA extensions on PCR products. The dU residues are converted to dT residues<i> in vivo</i> following transformation.

Artikel-Nr: (69750-3)

Lieferant: Merck Millipore (Novagen)

Beschreibung: The pET-24a-d(+) vectors carry an N-terminal T7-Tag® sequence plus an optional C-terminal His-Tag® sequence. These vectors differ from pET-21a-d(+) only by their selectable marker (kanamycin vs ampicillin resistance).

UOM: 1 * 10 µG

Aktion

Lieferant: Cytiva

Beschreibung: The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.

Artikel-Nr: (27-1542-01)

Lieferant: Cytiva

Beschreibung: The <i>E. coli </i>BL21 strain is protease-deficient for optimal expression of a variety of recombinant proteins.

UOM: 1 * 1 Vial

Aktion

Artikel-Nr: (PIER16152)

Lieferant: Thermo Fisher Scientific

Beschreibung: Green <i>Renilla</i> Luc vectors contain a gene cloned from the sea pansy, <i>Renilla reniformis</i>

UOM: 1 * 10 µG

Aktion

Lieferant: Thermo Fisher Scientific

Beschreibung: <i>Gaussia</i> Dura Luc-Vektoren enthalten eine mutierte Form des <i>Gaussia</i> Luciferase-Gens, das eine bessere Stabilität des Biolumineszenzsignals verleiht als native Luciferase. <i>Gaussia</i>-Dura-Luciferase (ca. 20 kDa) ist ein sezerniertes Protein, das die Messung der Reporteraktivität in Medien (für Echtzeit-Assays) und in Zelllysaten ermöglicht.

Artikel-Nr: (27-1524-01)

Lieferant: Cytiva

Beschreibung: M13 phage for the GST expression system.

UOM: 1 * 100 µl

Aktion

Lieferant: Thermo Fisher Scientific

Beschreibung: Red Firefly Luc vectors contains a mutant form of the firefly luciferase gene that has a red-shifted emission spectrum.

Lieferant: Thermo Fisher Scientific

Beschreibung: <i>Gaussia</i> Luc vectors contain a gene cloned from the copepod, <i>Gaussia princeps</i>. The gene encodes a naturally-secreted bioluminescent <i>Gaussia</i> luciferase (approx. 20 kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.

Artikel-Nr: (ICNA04821226)

Lieferant: MP Biomedicals

Beschreibung: pUC 18 is a small vector of approximately 2,7 Kb containing the Pvu II/EcoR I fragment of pBR322 and the lacZ gene multiple cloning site from M13mp18.

UOM: 1 * 25 µG

Aktion

Artikel-Nr: (ICNA04821225)

Lieferant: MP Biomedicals

Beschreibung: pBR322 is a 4363 bp vector purified from <i>Escherichia coli</i>. It is one of the most commonly used cloning vectors, that confers resistance to ampicillin and tetracycline. It contains single restriction sites for Pst I and Pvu I within the ampicillin-resistance gene (ampr) and single restriction sites for BamH I, Sal I, Sph I, EcoR V, Nru I and Xma III within the tetracycline-resistance gene (tetr).

UOM: 1 * 25 µG

Aktion

Lieferant: Cytiva

Beschreibung: Poly(A) (polyadenylic acid) can be used to evaluate the binding of potential therapeutic agents to mRNA in drug development research.

Zertifikate

Lieferant: Thermo Fisher Scientific

Beschreibung: <i>Cypridina</i> Luc vectors contain a gene cloned from the marine ostracod, <i>Cypridina noctiluca.</i>

Lieferant: Merck

Beschreibung: Polyadenylic acid (poly-A) is used to evaluate binding on cationic liposomes doped with non-ionic nucleolipids. Poly-A is used in small molecule mRNA targeted drug development to evaluate the binding of potential therapeutic agents such as the isoquinoline group of alkaloids.

Lieferant: biotechrabbit

Beschreibung: Expression vectors for PCR-based generation of linear templates.