Sie suchten nach: Proteinkonjugate und Markierungen

Lieferant: Biotium

Beschreibung: Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. It is non-fluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. It is useful for detecting reactive oxygen species including superoxide (in the presence of peroxidase or cytochrome c) and peroxynitrite. Dihydrorhodamine 123 dihydrochloride is functionally equivalent to dihydrorhodamine 123 but with increased stability toward air oxidation and light during storage. Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself has blue fluorescence (355/420 nm) in cells, while the oxidized form ethidium has red fluorescence (518/605 nm) upon DNA intercalation. H2DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H2DCFDA becomes the highly green fluorescent 2′,7′-dichlorofluorescein, 504/529 nm (end product).

Sicherheitsdatenblätter

Lieferant: G-Biosciences

Beschreibung: The micro HOOK™ Biotin kits are designed to label small amounts of proteins, with each kit designed for 8 to 10 labellings of 50 to 250 µg protein/reaction.

Artikel-Nr: (92224.)

Lieferant: Biotium

Beschreibung: This kit contains everything needed to label antibodies or other proteins with biotin succinimidyl ester (biotin SE). Each kit contains reagents for three labelling reactions of up to 1 mg protein per reaction.

UOM: 1 * 1 KIT

Aktion

Artikel-Nr: (COBSD4-000)

Lieferant: Columbia Biosciences

Beschreibung: Because of its high quantum yield, B-PE is considered the world’s brightest fluorophore.

UOM: 1 * 1 mg

Zertifikate Aktion

Lieferant: G-Biosciences

Beschreibung: HOOK™ IgG biotinylation kits are designed for the biotinylation of IgG molecules by immobilising the IgG molecules on a solid support. Nickel chelating resin allows for the rapid removal of uncoupled biotin and therefore eliminates the need for further dialysis or desalting of the biotinylated antibody.

Lieferant: Biotium

Beschreibung: Fluorescent CF® dye tyramides are used for tyramide signal amplification (TSA) for increasing immunofluorescence sensitivity in multicolour immunocytochemistry (ICC), immunohistochemistry (IHC), or <i>in situ</i> hybridization (ISH).

Lieferant: Biotium

Beschreibung: Near-IR Annexin V fluorescent conjugates can be used to detect apoptotic cells and is suitable for <i>in vivo</i> imaging.

Lieferant: Biotium

Beschreibung: Carbocyanine dyes with long hydrocarbon chains have low fluorescence in aqueous phase, but become highly fluorescent upon binding to lipid bilayers, making this class of dyes ideal for staining cell membranes. Carbocyanine dye membrane staining is very stable, with minimal dye transfer between cells. Dilinoleyl carbocynaine dyes have much faster lateral diffusion rates than regular carbocyanine dyes, which facilitates labelling of neurons in tissues.

Lieferant: Biotium

Beschreibung: A lectin labelled with our superior fluorescent CF® dyes is an effective and widely used cell surface stain for yeast, fungi, and mammalian cells.

Artikel-Nr: (786-404)

Lieferant: G-Biosciences

Beschreibung: CDI amine reactive agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates.

UOM: 1 * 10 mL

Aktion

Artikel-Nr: (ENZOENZ51032K100)

Lieferant: ENZO LIFE SCIENCES

Beschreibung: TheORGANELLE-ID-RGB® III assay kit contains Golgi apparatus-selective, endoplasmic reticulum-selective and nucleus-selective dyes suitable for live cell staining.

UOM: 1 * 1 KIT

Aktion

Artikel-Nr: (ENZOENZ42842L0050)

Lieferant: ENZO LIFE SCIENCES

Beschreibung: Orange 552 dUTP (lyophilised)

UOM: 1 * 50 Nmo

Aktion

Artikel-Nr: (ENZOENZ42842)

Lieferant: ENZO LIFE SCIENCES

Beschreibung: Orange 552 dUTP (5-TAMRA-dUTP) can replace TTP in reactions in which it serves as a substrate for <i>E. coli</i> DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Orange 552 dUTP can also be used for multicolor fluorescence labeling.

UOM: 1 * 25 Nmo

Aktion

Artikel-Nr: (92186.)

Lieferant: Biotium

Beschreibung: Biotin picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency.

UOM: 1 * 0,5 mg

Aktion

Lieferant: Biotium

Beschreibung: CF® dye-dCTP can be used to synthesise probes for in situ hybridisation, microarray, or to synthesise labelled DNA probes for in situ hybridisation and nucleic acid blotting applications.

Lieferant: Biotium

Beschreibung: CF™ dye azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalysed 1,3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with a phosphine containing compound.