Sie suchten nach: Protein-Extraktion

Lieferant: G-Biosciences

Beschreibung: Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT™ has been specifically developed for substantial removal of albumin from such samples.

Artikel-Nr: (786-022)

Lieferant: G-Biosciences

Beschreibung: FOCUS™ Mitochondria kit is specifically designed for the isolation of intact mitochondria from cultured mammalian cells. This kit allows for the fast and efficient fractionation of the cytoplasm of cultured mammalian cells into an enriched fraction of mitochondria. The majority (>90%) of the mitochondria have intact inner and outer membranes and therefore retain their functionality.

UOM: 1 * 1 KIT

Aktion

Artikel-Nr: (786-599)

Lieferant: G-Biosciences

Beschreibung: Immobilised Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non denaturing elution buffers.

UOM: 1 * 5 mL

Aktion

Artikel-Nr: (786-681)

Lieferant: G-Biosciences

Beschreibung: InGel™ Blue provides a complete set of reagents for the in-gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie® or fluorescent stained protein spots/bands.

UOM: 1 * 1 KIT

Aktion

Artikel-Nr: (786-793)

Lieferant: G-Biosciences

Beschreibung: Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave Immunoglobulin G (IgG) molecules in the hinge region.

UOM: 1 * 5 mL

Aktion

Lieferant: G-Biosciences

Beschreibung: Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG₁ Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.

Artikel-Nr: (786-598)

Lieferant: G-Biosciences

Beschreibung: Immobilised biotin resin is designed for the high affinity chromatography purifications of avidin, streptavidin and neutravidin protein. The resin consists of biotin coupled to 6% cross-linked agarose.

UOM: 1 * 5 mL

Aktion

Lieferant: ENZO LIFE SCIENCES

Beschreibung: Antigen retrieval reagent is used to recover antigens masked by fixation in cross linking fixatives such as formalin.

New Product

Artikel-Nr: (786-257)

Lieferant: G-Biosciences

Beschreibung: Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilises nearly all of the proteins from yeast, including membrane and soluble proteins. Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife™ Zymolyase®, which has improved stability and shelf life. Enzymatic action is followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.

UOM: 1 * 1 KIT

Aktion

Artikel-Nr: (786-244)

Lieferant: G-Biosciences

Beschreibung: SilverOUT removes interfering silver ions from gel pieces.

UOM: 1 * 100 Tests

Aktion

Artikel-Nr: (786-249)

Lieferant: G-Biosciences

Beschreibung: FOCUS™ Membrane Proteins for the fractionation of highly enriched membrane proteins.

UOM: 1 * 1 KIT

Aktion

Artikel-Nr: (DGA01)

Lieferant: G-Biosciences

Beschreibung: The 'critical micelle concentration' (CMC) of a detergent varies with temperature, pH, ionic strength, detergent concentration, purity and presence of organic agents in the detergent. Using a large excess of detergent may pose problems during purification procedures or other downstream applications.

UOM: 1 * 500 ST

Aktion

Lieferant: Thermo Fisher Scientific

Beschreibung: B-PER® Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells. The B-PER® Bacterial Protein Extraction Kit eliminates the need for harsh mechanical procedures, like sonication. The easy-to-use cell lysis solution uses mild non-ionic detergents to disrupt cells and solubilise proteins without denaturation. Enzyme supplements such as DNase I and Lysozyme allow for improved yield of large molecular weight proteins which are difficult to purify. B-PER® is also compatible with downstream protein purification methods such as GST purification and His-tagged immobilised metal affinity chromatography (IMAC).

Lieferant: G-Biosciences

Beschreibung: Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins and suppress peptide ionisation when analysed by mass spectrometry.

Zertifikate

Artikel-Nr: (786-018)

Lieferant: G-Biosciences

Beschreibung: FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membrane fractions are subsequently fractionated into either peripheral and integral membrane proteins or lipid raft associated proteins and detergent soluble membrane proteins.

UOM: 1 * 1 KIT

Aktion

Artikel-Nr: (PIER88279)

Lieferant: Thermo Fisher Scientific

Beschreibung: The Pierce™ Mouse Embryonic Fibroblast (MEF) Isolation Kit enables high yield isolation and culture of viable primary fibroblasts from mouse embryos.

UOM: 1 * 1 KIT

Aktion