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Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.


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Product Image-PIER88216

Micrococcal nuclease solution (≥100 U/µl)

Beschreibung: This Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.
Artikel-Nr: PIER88216
UOM: 1 * 1 ST
Lieferant: Thermo Fisher Scientific
Product Image-786-791

Immobilised Pepsin

Beschreibung: A proteolytic enzyme immobilised on 4% agarose that is routinely used for the generation of F(ab’)₂ fragments from Immunoglobulin G (IgG). The pepsin has the ability to cleave the heavy chains near the hinge region. The immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.
Artikel-Nr: 786-791
UOM: 1 * 1 ST
Lieferant: G-Biosciences
Product Image-786-790

Immobilised Papain

Beschreibung: A cysteine protease enzyme (EC 3.4.22.2) immobilised on 4% agarose, cleaves Immunoglobulin G (IgG) antibody molecules in the hinge region, generating three ~50 kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonisation, and lysis.
Artikel-Nr: 786-790
UOM: 1 * 1 ST
Lieferant: G-Biosciences
Product Image-PIER20308

SDS precipitation kit, SDS-Out™

Beschreibung: SDS-Out™ SDS Precipitation Kit provides convenient removal of SDS from small-volume (0,1 to 10 ml) protein samples.
Artikel-Nr: PIER20308
UOM: 1 * 1 KIT
Lieferant: Thermo Fisher Scientific
Product Image-PIER85113

Detergent solutions, Surfact-Amps™

Beschreibung: Surfact-Amps™ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques. These detergents are useful as a component of cell lysis buffers or other solutions intended to extract and solubilise proteins, improve chromatography, prepare yeast spheroplasts, and reduce background in ELISA or Western blot methods.
Artikel-Nr: PIER85113
UOM: 1 * 50 mL
Lieferant: Thermo Fisher Scientific
Product Image-PIER90058

Trypsin protease, MS grade

Beschreibung: Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.
Artikel-Nr: PIER90058
UOM: 1 * 1 KIT
Lieferant: Thermo Fisher Scientific

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Image Unavailable-324713-50

Endoproteinase Glu-C, excision grade, Staphylococcus aureus

Beschreibung: Native endoproteinase Glu-C from <i>Staphylococcus aureus</i>. Serine protease that specifically hydrolyses peptide bonds at the carboxylic side of glutamic acid and aspartic acid residues. Inhibited by DFP and α2-macroglobulin. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
Artikel-Nr: 324713-50
UOM: 1 * 50 µG
Lieferant: Merck Millipore (Calbiochem‎)
Image Unavailable-71092-3

PopCulture® reagent

Beschreibung: Das PopCulture® Reagenz ist ein auf Reinigungsmittel basiertes Konzentrat zur direkten Beimischung in E. coli-Kulturen, um ohne Zellernte wirksam Proteine extrahieren zu können. Rekombinante Proteine können direkt im Rohextrakt analysiert oder durch Hinzufügen einer Affinitätsmatrix, Auswaschen des gewünschten Proteinkomplexes aus der Matrix (zur Entfernung des verbrauchten Kulturmediums und von Zellverunreinigungen) und Elution des gereinigten Proteins aus der Matrix gereinigt werden. Das Proteinaufreinigungsverfahren mit PopCulture ist ideal zur automatisierten Verarbeitung großer Probenmengen in der Proteomikforschung sowie für jede andere Anwendung, für die eine höhere Geschwindigkeit und praktischere Vorgehensweisen sinnvoll sind.
Artikel-Nr: 71092-3
UOM: 1 * 15 mL
Lieferant: Merck Millipore (Novagen)
Image Unavailable-71456-4

Master mix, BugBuster®

Beschreibung: BugBuster® master mix combines BugBuster® protein extraction reagent with Benzonase® Nuclease and rLysozyme™ solution in one convenient reagent. It allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The master mix provides sufficient reagents for protein extraction from 20 g and 100 g cell paste.
Artikel-Nr: 71456-4
UOM: 1 * 500 mL
Lieferant: Merck Millipore (Novagen)
Product Image-71009-4

Protein extraction reagents, CytoBuster™

Beschreibung: The CytoBuster™ protein extraction reagent is a proprietary formulation of detergents optimised for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of the reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw.
Artikel-Nr: 71009-4
UOM: 1 * 250 mL
Lieferant: Merck Millipore (Novagen)
Image Unavailable-324708-2

Endoproteinase Asp-N, excision grade, Pseudomonas fragi

Beschreibung: Native endoproteinase Asp-N from <i>Pseudomonas fragi.</i> Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyses proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
Artikel-Nr: 324708-2
UOM: 1 * 2 µG
Lieferant: Merck Millipore (Calbiochem‎)
Product Image-786-680

Washing solution, Protein-OUT™

Beschreibung: Protein-OUT™ is a washing solution that cleans plastic and glassware used in the proteolytic digestion and peptide isolation for mass spectrometry analysis. The rinsing of plastic and glassware with Protein-OUT™ will remove interfering chemicals and peptides that can leach from the tube walls and interfere or suppress the mass spectrometry signal. In addition, rinsing with Protein-OUT™ removes inadvertent contamination with dust and keratin.
Artikel-Nr: 786-680
UOM: 1 * 50 mL
Lieferant: G-Biosciences
Product Image-REST25850

QuEChERS extraction salts, Q-sep®

Beschreibung: QuEChERS methods are fast, easy, and cost-effective, and Restek Q-sep® products make QuEChERS procedures even simpler.
Artikel-Nr: REST25850
UOM: 1 * 50 ST
Lieferant: Restek
Image Unavailable-VARIPL6010-4101

Uniform latex particles for immobilisation of biomolecules, PL-Latex

Beschreibung: Uniform latex particles are used in a wide range of applications that exploit their physico-chemical properties in sophisticated immunoassay formats, both in medicine and veterinary science research.
Artikel-Nr: VARIPL6010-4101
UOM: 1 * 1 ST
Lieferant: VARIAN
Image Unavailable-CEMATMS001-4X4L

TamiSolve® NxG-PS Solvents for Peptide Synthesis

Beschreibung: Modern peptide synthesis agents can dramatically reduce waste generation, but challenges remain regarding the health and environmental impacts of commonly used solvents.
Artikel-Nr: CEMATMS001-4X4L
UOM: 1 * 4 L
Lieferant: CEM

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